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Analysis of Microbe's from Municipal Waste Water (Pond) and Uses the Microbes for the Application of Waste Water Treatment

Received: 29 April 2021     Accepted: 10 July 2021     Published: 21 July 2021
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Abstract

Water contamination was a serious problem throughout the world. Various technologically advanced treatment methodologies for example activated sludge process, membrane bioreactor, trickling filter, rotating biological contractor and oxidation ditch are widely studied well documented and adopted in practice. However, attention to promising low-investment-cost technologies such as slow biological waste water treatments after preliminary/primary treatment techniques was quite effective water treatment technology. It is also efficient in removing coli form microorganisms such as Cryptosporidium, E. coli and bacteriophage from wastewater. Apart from reduction of pathogenic load which is ascribed to the biological processes. This way of technology adopted the methodology taking the sample in the field of water surface two parts in sampling pond water in the surface pond water and inner parts also handling two different glass bottles. Then in analysis their analysis the sample subjected to physiochemical analysis then again the sample subjected to serial dilution to identify the microbe’s spp in the water sample (surface and deepest) part of pond water surface part of sample more microbes familiar conducted in this experiment. Then by using those identified microbes dosing this is done using 10-6 and 10-5 by prepared nutrient agar (3.25 gram with 250 ml of distilled water). Then take 1% (0.5), 3% (1.5) and 5% (2.5) by volume of broth added into waste water of 49.5 ml, 48.5 ml and 47.5 ml respectively. This means that for 50 ml of conical flask as the colonies of culture increase the probability of waste water to be clear was too been high so 47.5 with 5% (2.5) combined form of broth high potent of cleaned.

Published in Chemical and Biomolecular Engineering (Volume 6, Issue 2)
DOI 10.11648/j.cbe.20210602.12
Page(s) 37-42
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Physiochemical Analysis, Biological Waste Water Treatment, Microbial Analysis, Pond Water, Nutrient Agar

References
[1] Asano, Y., Komeda. T. & Yamada. H. Microbial production of theobromine from caffeine. Bioscience Biotechnology Biochemistry, v. 57, p. 1286-1289, 1993.
[2] Blecher R, Lingens F. The metabolism of caffeine by a Pseudomonas putida strain. Hoppe-Seyler’s Z Physiol Chem. 1997; 358: 807-17.
[3] Buerge IJ, Poiger T, Muller MD, Buser HR. Caffeine, an anthropogenic marker for wastewater contamination of surface water, Environ Sci Technol, 2003; 37: 691-700.
[4] Camargo MCR, Toledo MCF Caffeine content of commercial Brazilian coffee Cienc Technol Aliment. 1998; 18: 421-4.
[5] Dash SS, Gummadi SN. Biodegradation of caffeine by Pseudomonas sp. NCIM 5235, Research Journal of Microbiology. 2010; 5 (8): 745-753.
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[7] Europaisches A. In: Coffeinum Theophyllinum, Deutscher Apotheker Verlag, Stuttgart; 1978, 670-121.
[8] Glassmeyer ST, Furlong ET, Kolpin DW, Cahill JD, Zaugg SD. Transport of chemical and microbial compounds from known waste water discharge: Potential for use as indicators of human fecal contamination, Environ Sci Technol, 2005; 39: 5157-5169.
[9] Mussatto, S. I., & Roberto, I. C. (2005). Acid hydrolysis and fermentation of brewer‟s spent grain to produce xylitol. Journal of the Science of Food and Agriculture, 85, 2453–2460.
[10] Mussatto, S. I., & Teixeira, J. A. (2010). Increase in the fructooligosaccharides yield and productivity by solid-state fermentation with Aspergillus japonicus using agro-industrial residues as support and nutrient source. Biochemical Engineering Journal, 53, 154–157.
[11] Punj S, editors. Abiotic stress: new research. Hauppauge, NY: Nova science publishers Inc: 2012. pp. 1-57.
[12] Rawel, H. M., & Kulling, S. E. (2007). Nutritional contribution of coffee, cacao and tea phenolics to human health. Journal of Consumer Protection and Food Safety, 2, 399–406.
[13] R. Losco and J. Valentine, “Storm water Infiltration and the Soil Landscape Connection,” Pennsylvania Association of Professional Soil Scientists. 2003.
[14] http://www3.villanova.edu/vusp/Outreach/pasym03/pdfs/ 3A3.pdf
[15] Salihu Ibrahim, Mohd Yunus Shukor, Mohd Arif Syed, Nor Arina Ab Rahman, Khalilah Abdul Khalil, Ariff Khalid et al. Bacterial degradation of caffeine: a Review. Asian Journal of Plant Biology. 2014; 2 (1): 18-27.
[16] S. Clark and R. Pitt, “Storm water Runoff Treatment: Evaluation of Filtration Media,” US Environmental Protection Agency, Water Supply and Water Resources Division, National Risk Management Research Laboratory, EPA No. 600R00010, Cincinnati, 1999.
[17] Nutrient management system. Can J Microbiol. 2008: 54; 876-886. doi: 10. 1139/W08-081. [PubMed] [Cross_Ref].
[18] International Organization for Standardization (ISO) Standard 9000: 2005. Quality management system-fundamental and vocabulary. ISO, Switzerland, pp 1.
[19] https://en.wikipedia.org/wiki/Bacteria
[20] http://www.countyofdane.com/lwrd/landconservation/papers/impactsofswinfiltration.pdf
[21] Wang, D., Sakoda, A., & Suzuki, M. (2001). Biological efficiency and nutritional value of Pleurotus ostreatus cultivated on spent beer grain. Bioresource Technology, 78, 293–300.
[22] Yamoka-Yano DM, Mazzafera P. Degradation of caffeine by pseudomonas putida isolated from soil. Allel J 1998; 5: 23-34.
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Cite This Article
  • APA Style

    Zeynu Shamil Awol, Rezika Tofike Abate. (2021). Analysis of Microbe's from Municipal Waste Water (Pond) and Uses the Microbes for the Application of Waste Water Treatment. Chemical and Biomolecular Engineering, 6(2), 37-42. https://doi.org/10.11648/j.cbe.20210602.12

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    ACS Style

    Zeynu Shamil Awol; Rezika Tofike Abate. Analysis of Microbe's from Municipal Waste Water (Pond) and Uses the Microbes for the Application of Waste Water Treatment. Chem. Biomol. Eng. 2021, 6(2), 37-42. doi: 10.11648/j.cbe.20210602.12

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    AMA Style

    Zeynu Shamil Awol, Rezika Tofike Abate. Analysis of Microbe's from Municipal Waste Water (Pond) and Uses the Microbes for the Application of Waste Water Treatment. Chem Biomol Eng. 2021;6(2):37-42. doi: 10.11648/j.cbe.20210602.12

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  • @article{10.11648/j.cbe.20210602.12,
      author = {Zeynu Shamil Awol and Rezika Tofike Abate},
      title = {Analysis of Microbe's from Municipal Waste Water (Pond) and Uses the Microbes for the Application of Waste Water Treatment},
      journal = {Chemical and Biomolecular Engineering},
      volume = {6},
      number = {2},
      pages = {37-42},
      doi = {10.11648/j.cbe.20210602.12},
      url = {https://doi.org/10.11648/j.cbe.20210602.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cbe.20210602.12},
      abstract = {Water contamination was a serious problem throughout the world. Various technologically advanced treatment methodologies for example activated sludge process, membrane bioreactor, trickling filter, rotating biological contractor and oxidation ditch are widely studied well documented and adopted in practice. However, attention to promising low-investment-cost technologies such as slow biological waste water treatments after preliminary/primary treatment techniques was quite effective water treatment technology. It is also efficient in removing coli form microorganisms such as Cryptosporidium, E. coli and bacteriophage from wastewater. Apart from reduction of pathogenic load which is ascribed to the biological processes. This way of technology adopted the methodology taking the sample in the field of water surface two parts in sampling pond water in the surface pond water and inner parts also handling two different glass bottles. Then in analysis their analysis the sample subjected to physiochemical analysis then again the sample subjected to serial dilution to identify the microbe’s spp in the water sample (surface and deepest) part of pond water surface part of sample more microbes familiar conducted in this experiment. Then by using those identified microbes dosing this is done using 10-6 and 10-5 by prepared nutrient agar (3.25 gram with 250 ml of distilled water). Then take 1% (0.5), 3% (1.5) and 5% (2.5) by volume of broth added into waste water of 49.5 ml, 48.5 ml and 47.5 ml respectively. This means that for 50 ml of conical flask as the colonies of culture increase the probability of waste water to be clear was too been high so 47.5 with 5% (2.5) combined form of broth high potent of cleaned.},
     year = {2021}
    }
    

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  • TY  - JOUR
    T1  - Analysis of Microbe's from Municipal Waste Water (Pond) and Uses the Microbes for the Application of Waste Water Treatment
    AU  - Zeynu Shamil Awol
    AU  - Rezika Tofike Abate
    Y1  - 2021/07/21
    PY  - 2021
    N1  - https://doi.org/10.11648/j.cbe.20210602.12
    DO  - 10.11648/j.cbe.20210602.12
    T2  - Chemical and Biomolecular Engineering
    JF  - Chemical and Biomolecular Engineering
    JO  - Chemical and Biomolecular Engineering
    SP  - 37
    EP  - 42
    PB  - Science Publishing Group
    SN  - 2578-8884
    UR  - https://doi.org/10.11648/j.cbe.20210602.12
    AB  - Water contamination was a serious problem throughout the world. Various technologically advanced treatment methodologies for example activated sludge process, membrane bioreactor, trickling filter, rotating biological contractor and oxidation ditch are widely studied well documented and adopted in practice. However, attention to promising low-investment-cost technologies such as slow biological waste water treatments after preliminary/primary treatment techniques was quite effective water treatment technology. It is also efficient in removing coli form microorganisms such as Cryptosporidium, E. coli and bacteriophage from wastewater. Apart from reduction of pathogenic load which is ascribed to the biological processes. This way of technology adopted the methodology taking the sample in the field of water surface two parts in sampling pond water in the surface pond water and inner parts also handling two different glass bottles. Then in analysis their analysis the sample subjected to physiochemical analysis then again the sample subjected to serial dilution to identify the microbe’s spp in the water sample (surface and deepest) part of pond water surface part of sample more microbes familiar conducted in this experiment. Then by using those identified microbes dosing this is done using 10-6 and 10-5 by prepared nutrient agar (3.25 gram with 250 ml of distilled water). Then take 1% (0.5), 3% (1.5) and 5% (2.5) by volume of broth added into waste water of 49.5 ml, 48.5 ml and 47.5 ml respectively. This means that for 50 ml of conical flask as the colonies of culture increase the probability of waste water to be clear was too been high so 47.5 with 5% (2.5) combined form of broth high potent of cleaned.
    VL  - 6
    IS  - 2
    ER  - 

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Author Information
  • Department of Chemical Engineering, College of Engineering and Technology, Wolkite University, Wolkite, Ethiopian

  • Department of Chemical Engineering, College of Engineering and Technology, Wolkite University, Wolkite, Ethiopian

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